University of Florida - Flow Cytometry Laboratory Analysis USA

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[ Visit Site ] The Flow Cytometry Core Laboratory at the University of Florida will accept samples from outside organizations and companies. A representative (usually the researcher) should contact the Scientific Director in advance to discuss specific requirements and how we will bill you, handle and analyze your samples, and provide your data.

Equipment:
* The FACScan is the most basic instrument we have. It can analyze forward and side light scatter, and up to 3 colors (green, orange, and red) of fluorescence. It has a single laser that emits 488 nm (blue) light.
* The FACSort is similar to the FACScan. In addition to the above analysis functions, it adds low speed (<300 cells/second) sorting capability. Since cells are totally contained during the sort process, it is often used for sorting small numbers of cells under BSL-2 conditions.
* The FACSCalibur is similar to the FACScan, but it adds a 2nd laser that emits 635 nm (red) light and a 4th fluorescence detector, so it can analyze more fluorescent dyes simultaneously. We have 2 FACS Calibur instruments; one is equipped with an automatic sample tube changer.
* The CyAn contains 3 lasers (blue, red, and violet) and can measure forward and side scatter, and up to 9 colors of fluorescence. The CyAn is appropriate for work utilizing more than 4 fluorochromes, or work involving fluorochromes that, due to their uncommon spectral characteristics, can not be analyzed on the other instruments.
* The FACS Vantage SE Turbosort contains blue and red lasers, as well as a krypton laser that is tunable to either violet (413 or 407) or ultra-violet. It can measure forward and side scatter, and up to 8 colors of fluorescence. The major function of this instrument is sorting, or separating, cell populations at high-speed (10,000 cells/second or more). The DiVa option adds digital signal processing electronics, improving overall speed and purity of the sorted populations.

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